Molecular identification of Mycobacterium avium subspecies paratuberculosis in oral biopsies of Crohn’s disease patients
نویسندگان
چکیده
Oral lesions may be found in patients with Crohn's disease (CD), in a percentage up to 20%. The aim of this study was to investigate a possible relationship between Mycobacterium avium subsp. paratuberculosis (MAP) and oral lesions in CD patients. 23 oral biopsies were examined performing IS900 Nested PCR; 9 of them were positive: 8 from CD patients and 1 from a control. Our purpose is to go on with this study, amplifying the number of subjects examined and testing subjects with oral lesions related to diseases other than CD to verify the specific association between MAP and oral lesions in CD patients.
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Genomic Detection of Mycobacterium Avium Subspecies Paratuberculosis in Blood Samples of Patients with Inflammatory Bowel Disease in Southern Iran
Background: Inflammatory bowel disease (IBD), of which Crohn’s disease (CD) and ulcerative colitis (UC) are the two main clinicopathological subtypes, is a group of digestive system diseases of unknown etiology. Risk factors for IBD are environmental factors, genetics, and immune system agents. Mycobacterium avium subspecies paratuberculosis (MAP) is one of the most important infectious factors...
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Figure 1. Representative PCR results showing amplification (or otherwise) of 298-bp genomic fragment corresponding to IS900 in diabetic patients (lanes 1–8). Lanes 9 and 10, Control subjects without type 1 diabetes mellitus. Lanes 11 and 12, Mycobacterium avium subspecies paratuberculosis–positive and M. avium subspecies paratuberculosis–negative control subjects, respectively. M, molecular mar...
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